LOCUS       sequence-72999-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contributes the coding sequence for EGFP as the 5'-module during 
            MultiSite Gateway-cloning of chimeric cDNAs encoding three-part 
            fusion proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Buj R, Iglesias N, Planas AM, Santalucia T
  TITLE     A plasmid toolkit for cloning chimeric cDNAs encoding customized 
            fusion proteins into any Gateway destination expression vector.
  JOURNAL   BMC Mol Biol. 2013 Aug 20;14(1):18. doi: 10.1186/1471-2199-14-18.
  PUBMED    23957834
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Mol 
            Biol."; date: "2013-08-20"; volume: "14(1):18. doi"; pages: " 
            10.1186/1471-2199-14-18"
COMMENT     Sequence Label: pDONR P4-P1R-EGFP
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..102
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      102..111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(108..110,111..113,114..821)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKVSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(153..174)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(414..433)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     761..782
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    822..946
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 agctccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgata
       61 agcaatgctt ttttataatg ccaactttgt atagaaaagt tgccaccatg gtgagcaagg
      121 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      181 gccacaaggt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      241 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      301 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
      361 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      421 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      481 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      541 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
      601 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
      661 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
      721 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
      781 tgaccgccgc cgggatcact ctcggcatgg acgagctgta cacaagtttg tacaaaaaag
      841 ttgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa
      901 aacagactac ataatactgt aaaacacaac atatgcagtc actatgaacc a
//
