LOCUS       sequence-73002-e        1075 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA-Sumo2Q90P mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Bekes M, Prudden J, Srikumar T, Raught B, Boddy MN, Salvesen GS
  TITLE     The dynamics and mechanism of SUMO chain deconjugation by 
            SUMO-specific proteases.
  JOURNAL   J Biol Chem. 2011 Mar 25;286(12):10238-47. Epub 2011 Jan 19.
  PUBMED    21247896
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2011-03-25"; volume: "286(12)"; pages: "10238-47. 
            Epub 2011 Jan 19"
COMMENT     Sequence Label: pcDNA3 HA-SUMO2-Q90P
FEATURES             Location/Qualifiers
     source          1..1075
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             102..128
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             126..401
                     /codon_start=1
                     /product="human small ubiquitin-related modifier 2"
                     /label=SUMO2
                     /translation="ADEKPKEGVKTENNDHINLKVAGQDGSVVQFKIKRHTPLSKLMKA
                     YCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQPTGG"
     promoter        complement(432..450)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(433..450)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(470..487)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    476..700
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(833..852)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1043..1064
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttatggatgc ctacccatac gacgtaccag
      121 actacgccga cgaaaagccc aaggaaggag tcaagactga gaacaacgat catattaatt
      181 tgaaggtggc ggggcaggat ggttctgtgg tgcagtttaa gattaagagg catacaccac
      241 ttagtaaact aatgaaagcc tattgtgaac gacagggatt gtcaatgagg cagatcagat
      301 tccgatttga cgggcaacca atcaatgaaa cagacacacc tgcacagttg gaaatggagg
      361 atgaagatac aattgatgtg ttccaacagc ctacgggagg ttgactcgag catgcatcta
      421 gagggcccta ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg
      481 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
      541 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
      601 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
      661 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
      721 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
      781 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
      841 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
      901 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
      961 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     1021 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actca
//
