LOCUS       sequence-73003-e        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MYC-Sumo3Q90P mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Bekes M, Prudden J, Srikumar T, Raught B, Boddy MN, Salvesen GS
  TITLE     The dynamics and mechanism of SUMO chain deconjugation by 
            SUMO-specific proteases.
  JOURNAL   J Biol Chem. 2011 Mar 25;286(12):10238-47. Epub 2011 Jan 19.
  PUBMED    21247896
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2011-03-25"; volume: "286(12)"; pages: "10238-47. 
            Epub 2011 Jan 19"
COMMENT     Sequence Label: pcDNA3 MYC-Sumo3Q89P
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             135..404
                     /codon_start=1
                     /product="human small ubiquitin-related modifier 3"
                     /label=SUMO3
                     /translation="EEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYC
                     ERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQPTGG"
     promoter        complement(435..453)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(436..453)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(473..490)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    479..703
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(836..855)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1046..1067
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttatggatgc cgagcagaag ctgatctcag
      121 aagaagatat cgccgaggag aagcccaagg agggtgtgaa gacagagaat gaccacatca
      181 acctgaaggt ggccgggcag gacggctccg tggtgcagtt caaaatcaag aggcacacgc
      241 cgctgagcaa gctgatgaag gcctactgcg agaggcaggg cttgtcaatg aggcagatca
      301 gattcaggtt cgacgggcag ccaatcaatg aaactgacac tccagcacag ctggagatgg
      361 aggacgagga caccatcgac gtgttccagc agccaacggg aggttgactc gagcatgcat
      421 ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact
      481 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
      541 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
      601 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
      661 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
      721 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
      781 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
      841 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
      901 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
      961 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     1021 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aac
//
