LOCUS       sequence-73010-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contributes the coding sequence for ECFP as the 5'-module during 
            MultiSite Gateway-cloning of chimeric cDNAs encoding three-part 
            fusion proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Buj R, Iglesias N, Planas AM, Santalucia T
  TITLE     A plasmid toolkit for cloning chimeric cDNAs encoding customized 
            fusion proteins into any Gateway destination expression vector.
  JOURNAL   BMC Mol Biol. 2013 Aug 20;14(1):18. doi: 10.1186/1471-2199-14-18.
  PUBMED    23957834
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Mol 
            Biol."; date: "2013-08-20"; volume: "14(1):18. doi"; pages: " 
            10.1186/1471-2199-14-18"
COMMENT     Sequence Label: pDONR P4-P1R-ECFP
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..116
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      116..125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(122..124,125..127,128..835)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(167..188)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(428..447)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     775..796
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    836..860
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttacgctacc atggagctcc aaataatgat tttattttga ctgatagtga cctgttcgtt
       61 gcaacaaatt gataagcaat gcttttttat aatgccaact ttgtatagaa aagttgccac
      121 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      181 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      241 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      301 cctcgtgacc accctgacct ggggcgtgca gtgcttcagc cgctaccccg accacatgaa
      361 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      421 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      481 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      541 caagctggag tacaactaca tcagccacaa cgtctatatc accgccgaca agcagaagaa
      601 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
      661 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      721 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
      781 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacacaag
      841 tttgtacaaa aaagttgaac gagaaacgta aaatgatata aatatcaata tattaaatta
      901 gattttgcat aaaaaacaga ctacataata ctgtaaa
//
