LOCUS       sequence-73012-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contributes an IRES-ECFP cassette as the 3'-module during MultiSite 
            Gateway cloning of a bi-cistronic mRNA shared with a two-part fusion
            protein encoded by the 5′- and middle modules..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Buj R, Iglesias N, Planas AM, Santalucia T
  TITLE     A plasmid toolkit for cloning chimeric cDNAs encoding customized 
            fusion proteins into any Gateway destination expression vector.
  JOURNAL   BMC Mol Biol. 2013 Aug 20;14(1):18. doi: 10.1186/1471-2199-14-18.
  PUBMED    23957834
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Mol 
            Biol."; date: "2013-08-20"; volume: "14(1):18. doi"; pages: " 
            10.1186/1471-2199-14-18"
COMMENT     Sequence Label: pDONR P2R-P3-IRES_ECFP
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(54..177)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    join(216..790,791..793,794..802)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(383..400)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     610..629
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 cagctctaga gctcgaattc tacaggtcac taataccatc taagtagttg gttcatagtg
       61 actgcatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
      121 atatattgat atttatatca ttttacgttt ctcgttcaac tttcttgtac aaagtggaca
      181 ttctgcagtc gacggtaccg cgggcccggg atccgcccct ctccctcccc cccccctaac
      241 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
      301 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
      361 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
      421 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
      481 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
      541 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
      601 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
      661 ccccattgta tgggatctga tctggggcct cggtacacat gctttacatg tgtttagtcg
      721 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
      781 cgatgataat atggccacaa ccatggtgag caagggcgag gagctgttca ccggggtggt
      841 gcccatcctg gtcga
//
