LOCUS       sequence-73013-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contributes a cassette containing an SV40 polyA signal as the 
            3'-module during MultiSite Gateway cloning of chimeric cDNAs, when a
            peptide module at the C-terminal end is not required..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Buj R, Iglesias N, Planas AM, Santalucia T
  TITLE     A plasmid toolkit for cloning chimeric cDNAs encoding customized 
            fusion proteins into any Gateway destination expression vector.
  JOURNAL   BMC Mol Biol. 2013 Aug 20;14(1):18. doi: 10.1186/1471-2199-14-18.
  PUBMED    23957834
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Mol 
            Biol."; date: "2013-08-20"; volume: "14(1):18. doi"; pages: " 
            10.1186/1471-2199-14-18"
COMMENT     Sequence Label: pDONR P2R-P3-SV40 polyA signal
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(54..177)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    180..301
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(217..236)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     271..290
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(312..407)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(444..463)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(445..463)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(468..484)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(468..484)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(586..605)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(664..683)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 cagctctaga gctcgaattc tacaggtcac taataccatc taagtagttg gttcatagtg
       61 actgcatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
      121 atatattgat atttatatca ttttacgttt ctcgttcaac tttcttgtac aaagtggaca
      181 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
      241 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
      301 atcatgtcgg acaactttat tatacaaagt tggcattata aaaaagcatt gcttatcaat
      361 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgga gctccatggt
      421 agcgttaacg cggccgcgat atcccctata gtgagtcgta ttacatggtc atagctgttt
      481 cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
      541 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga
      601 gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat
      661 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt
      721 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
      781 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
      841 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggaa
      901 aa
//
