LOCUS       sequence-73015-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of N-terminally tagged Sumo1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  TITLE     Addgene's unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJ609 Flag-Sumo1
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             102..125
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(470..487)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    476..587
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(705..725)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 tagcgagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata ggggacaccc aagctggcta gcgacaccat ggactacaaa gacgatgacg
      121 acaagggtcc aggacctggt ccaggaccta tgtctgacca ggaggcaaaa ccttcaactg
      181 aggacttggg ggataagaag gaaggtgaat atattaaact caaagtcatt ggacaggata
      241 gcagtgagat tcacttcaaa gtgaaaatga caacacatct caagaaactc aaagaatcat
      301 actgtcaaag acagggtgtt ccaatgaatt cactcaggtt tctctttgag ggtcagagaa
      361 ttgctgataa tcatactcca aaagaactgg gaatggagga agaagatgtg attgaagttt
      421 atcaggaaca aacggggggt cattcaacag tttagctcga gaaaatcagc ctcgactgtg
      481 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
      541 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcaca acactcaacc
      601 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
      661 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
      721 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      781 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      841 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      901 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt t
//
