LOCUS       sequence-73018-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Kowalska E, Ripperger JA, Muheim C, Maier B, Kurihara Y, Fox AH, 
            Kramer A, Brown SA
  TITLE     Distinct roles of DBHS family members in the circadian 
            transcriptional feedback loop.
  JOURNAL   Mol Cell Biol. 2012 Nov;32(22):4585-94. doi: 10.1128/MCB.00334-12. 
            Epub 2012 Sep 10.
  PUBMED    22966205
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00334-12"; journalName: "Mol Cell Biol"; date: 
            "2012-11"; volume: "32"; issue: "22"; pages: "4585-94"
COMMENT     Sequence Label: pSCT-GAL93-DEST
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(95..101,102..119,120..125))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(150..274)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(605..624)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(606..624)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(652..855)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(681..701)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gggaaatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
       61 gaaaatctcg acggatccta actcaaaatc cacacattat acgagccgga agcataaagt
      121 gtaaagcctg gggtgcctaa tgcggccgcc atagtgactg gatatgttgt gttttacagt
      181 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
      241 acgtttctcg ttcagctttt ttgtacaaac ttgttgatcc tgcaggggta cccaacaatg
      301 cttttatatc ctgtaaagaa tccattttca aaatcatgtc aaggtcttct cgaggaaaaa
      361 tcagtagaaa tagctgttcc agtctttcta gccttgattc cacttctgtc agatgtgccc
      421 tagtcagcgg agaccttttg gttttgggag agtagcgaca ctcccagttg ttcttcagac
      481 acttggcgca cttcggtttt tctttggagc acttgagctt tttaagtcgg caaatatcgc
      541 atgcttgttc gatagaagac agtagcttca tctttcagga ggcttgcttc aagcttagat
      601 ccgtccctat agtgagtcgt attaccaggc gatctgacgg ttcactaaac cagctctgct
      661 tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt
      721 acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat
      781 ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg
      841 ccaaaaccgc atcaccatgg taatagcgat
//
