LOCUS       sequence-73021-e        1131 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses catalytic domain of MALT1 catalytic mutant in e.coli AA 
            329-566.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Hachmann J, Snipas SJ, van Raam BJ, Cancino EM, Houlihan EJ, Poreba 
            M, Kasperkiewicz P, Drag M, Salvesen GS
  TITLE     Mechanism and specificity of the human paracaspase MALT1.
  JOURNAL   Biochem J. 2012 Apr 1;443(1):287-95. doi: 10.1042/BJ20120035.
  PUBMED    22309193
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochem J"; date: "2012-04-1- 1"; volume: "443"; issue: "1"; pages:
            "287-95"
COMMENT     Sequence Label: pET21 Catalytic Domain of MALT1 Catalytic Mutant
FEATURES             Location/Qualifiers
     source          1..1131
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..56)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             799..821
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    836..860
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(860..879)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(861..879)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(963..982)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tcagcttcct ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg
       61 agtccctgta ttggatcagt aagtgctctc ttctcagata aactacttcg aatctctaga
      121 gcctgtttgc ctttggtaag gtgacactta cccatatctt ctgcaacttc atccagtaac
      181 acagtgattt tcttatcttc taataatctg tcttttaaaa atttcataaa gattccattt
      241 gccaatccag aatgctggat ttcaaaagct tctgctcctt gacacgtggc atatccaaac
      301 acaatattgg cggtgacttt tagtgcatcc aagattggaa tggtatcatc gtagtcattt
      361 cttttcctag ccatatccaa taagaacaca ttaagtccag tttctttttc ttgcatcaat
      421 ttcagtatat tttgtacaca cagacagttt tcagacctat atggatttgg agcatcaacg
      481 gggaccatga agctgttccc aaaattttcg taaccatgtc ctgcataata taataaccca
      541 tatactccct tgtctaaaag gagtaaaaac tcatccacag cattacgcat ctcatattca
      601 gtaagatcca acagtgaaac cactttgaag tccagctgtc tcagtaagtt agtcaattcg
      661 tacacatcca ccaaaggagc tttgagcttg gggtgctccc ggtaattcat atttcctatc
      721 aaaagggcaa ccttgtcctt cgccaaaggc tggtcagttg tttgctcttt attatcgaat
      781 tcgctagcca tatgtatatc tccttcttaa agttaaacaa aattatttct agaggggaat
      841 tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatctc
      901 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
      961 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
     1021 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
     1081 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc t
//
