LOCUS       sequence-73023-e        1064 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Kitamura T, Kitamura YI, Funahashi Y, Shawber CJ, Castrillon DH, 
            Kollipara R, DePinho RA, Kitajewski J, Accili D
  TITLE     A Foxo/Notch pathway controls myogenic differentiation and fiber 
            type specification.
  JOURNAL   J Clin Invest. 2007 Sep . 117(9):2477-85.
  PUBMED    17717603
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2007 Sep . 117(9):2477-85."
COMMENT     Sequence Label: DBD (N208AH212R) Foxo1-WT pDNR-CMV
FEATURES             Location/Qualifiers
     source          1..1064
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..46
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(105..138)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             150..173
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg taaaacgacg gccagtagat ctgtaatacg
       61 actcactata gggcgctagc gtcagtgagc gaggaagcgg ccgcataact tcgtatagca
      121 tacattatac gaagttatca gtcgacatgg actacaagga cgacgatgac aaggccgaag
      181 cgccccaggt ggtggagacc gacccggact tcgagccgct gccccggcag cgctcctgta
      241 cctggccgct gcccaggccg gagtttaacc agtccaactc gaccacctcc agtccggcgc
      301 cgtcgggcgg cgcggccgcc aaccccgacg ccgcggcgag cctggcctcg gcgtccgctg
      361 tcagcaccga ctttatgagc aacctgagcc tgctggagga gagtgaggac ttcgcgcggg
      421 cgccaggctg cgtggccgtg gcggcggcgg ctgcggccag caggggcctg tgcggggact
      481 tccagggccc cgaggcgggc tgcgtgcacc cagcgccgcc acagccccca ccgacctggc
      541 cgctgtcgca gcccccaccc gtgcctccct ccgctgccgc cgccgcgggg ccactcgcgg
      601 gacagccgcg caagaccagc tcgtcgcgcc gcaacgcgtg gggcaacctg tcgtacgccg
      661 acctcatcac caaggccatc gagagctcag ccgagaagag gctcaccctg tcgcagatct
      721 acgagtggat ggtgaagagc gtgccctact tcaaggataa gggcgacagc aacagctcgg
      781 cgggctggaa ggcttcaatt cgccgcaatc tgtcccttca cagcaggttt attcgagtgc
      841 agaatggagg aactggaaag agttcttggt ggatgctcaa tccagaggga ggcaagagcg
      901 gaaaatcacc ccggagaaga gctgcgtcca tggacaacaa cagtaaattt gctaagagcc
      961 gagggcgggc tgctaagaaa aaagcatctc tccagtctgg gcaagagggt cctggagaca
     1021 gccctgggtc tcagttttct aagtggcctg cgagtcctgg gtcc
//
