LOCUS       sequence-73028-e         671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent viral expression of oChIEF-tdTomato for optogenetic 
            excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Bell KA, Shim H, Chen CK, McQuiston AR
  TITLE     Nicotinic excitatory postsynaptic potentials in hippocampal CA1 
            interneurons are predominantly mediated by nicotinic receptors that 
            contain alpha4 and beta2 subunits.
  JOURNAL   Neuropharmacology. 2011 Aug 25.
  PUBMED    21878344
REFERENCE   2  (bases 1 to 671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuropharmacology. 2011 Aug 25."
COMMENT     Sequence Label: AAV-Flex-rev-oChIEF-tdTomato
FEATURES             Location/Qualifiers
     source          1..671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     201..218
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    335..356
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(371..376,377..394,395..401)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    409..425
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     414..436
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     433..449
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     433..449
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        467..485
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     467..484
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    492..548
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(550..569)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(551..569)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(576..593)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(576..592)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(585..607)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
       61 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      121 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      181 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      241 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgcgcg cgnangtttc
      301 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
      361 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
      421 aacaatttca cacaggaaac agctatgacc atgattacgc caagctattt aggtgacact
      481 atagaatact caagcttgca tgcctgcagg tcgactctag aggatccccg ggtaccgagc
      541 tcgaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt
      601 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
      661 agctggcgta a
//
