LOCUS       sequence-73034-e        1093 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   de Felippes FF, Wang JW, Weigel D
  TITLE     MIGS: MiRNA Induced Gene Silencing.
  JOURNAL   Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x.
  PUBMED    22211571
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x."
COMMENT     Sequence Label: MIGS2.2 (FF573)
FEATURES             Location/Qualifiers
     source          1..1093
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(124..248)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      255..896
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(918..938)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(918..936)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(957..973)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(957..973)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(970..992)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    981..997
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1005..1011,1012..1029,1030..1035))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1050..1071
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
       61 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcacctc
      121 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
      181 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
      241 aaagtggtga tcctagcttt cgttcgtatc atcggtttcg acaacgttcg tcaagttcaa
      301 tgcatcagtt tcattgcgca cacaccagaa tcctactgag tttgagtatt atggcattgg
      361 gaaaactgtt tttcttgtac catttgttgt gcttgtaatt tactgtgttt tttattcggt
      421 tttcgctatc gaactgtgaa atggaaatgg atggagaaga gttaatgaat gatatggtcc
      481 ttttgttcat tctcaaatta atattatttg ttttttctct tatttgttgt gtgttgaatt
      541 tgaaattata agagatatgc aaacattttg ttttgagtaa aaatgtgtca aatcgtggcc
      601 tctaatgacc gaagttaata tgaggagtaa aacacttgta gttgtaccat tatgcttatt
      661 cactaggcaa caaatatatt ttcagaccta gaaaagctgc aaatgttact gaatacaagt
      721 atgtcctctt gtgttttaga catttatgaa ctttccttta tgtaattttc cagaatcctt
      781 gtcagattct aatcattgct ttataattat agttatactc atggatttgt agttgagtat
      841 gaaaatattt tttaatgcat tttatgactt gccaattgat tgacaacatg catcaatcgg
      901 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
      961 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     1021 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     1081 cgctcactgc ccg
//
