LOCUS       sequence-73036-e        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   de Felippes FF, Wang JW, Weigel D
  TITLE     MIGS: MiRNA Induced Gene Silencing.
  JOURNAL   Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x.
  PUBMED    22211571
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x."
COMMENT     Sequence Label: MIGS2.2 (FF573)
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(75..254)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     486..508
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     500..517
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     501..517
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     527..546
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        527..545
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     570..586
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     571..587
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        617..635
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     617..634
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcctcgatc gagttgagag
       61 tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt ggagcatttt
      121 tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg cgcaataatg
      181 gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctca gtggctcctt
      241 caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg
      301 gtcataacgt gactccctta attctccgct catgatcgat aacattaacg cttacaattt
      361 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
      421 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      481 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact
      541 atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ggtatcgata agcttgatat
      601 cgaattcctg cagcccattt aggtgacact atagaatatc actagtaagc tagcttgcat
      661 gccggtcctg ctgagcctcg acatgttgtc gcaaaattcg ccctggaccc gcccaacgat
      721 ttgtcgtcac tgtcaaggtt tgacctgcac ttcatttggg gcccacatac accaaaaaaa
      781 tgctgcataa ttctcggggc agcaagtcgg ttacccggcc gccgtgctgg accgggttga
      841 atggtgcccg taactttcgg tagagcggac ggccaatact caacttcaag gaatctcacc
      901 catgcgcgcc ggcggggaac cggagttccc ttcagtgagc gttattagtt cgccgctcgg
      961 tgtgtcgtag atactagccc ctggggcact tttgaaattt gaataagatt tatgtaatca
     1021 gtcttttagg tttgaccggt tctgccgctt tttttaaaat tggatttgta ataataaaac
     1081 gcaattgttt gttattgtgg
//
