LOCUS       sequence-73037-e         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   de Felippes FF, Wang JW, Weigel D
  TITLE     MIGS: MiRNA Induced Gene Silencing.
  JOURNAL   Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x.
  PUBMED    22211571
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x."
COMMENT     Sequence Label: MIGS2.2 (FF573)
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     44..60
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     45..61
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        91..109
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     91..108
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      complement(142..849)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt cgacggtatc
       61 gataagcttg atatcgaatt cctgcagccc atttaggtga cactatagaa tatcactagt
      121 aagctagctt gcatgccggt cctgctgagc ctcgacatgt tgtcgcaaaa ttcgccctgg
      181 acccgcccaa cgatttgtcg tcactgtcaa ggtttgacct gcacttcatt tggggcccac
      241 atacaccaaa aaaatgctgc ataattctcg gggcagcaag tcggttaccc ggccgccgtg
      301 ctggaccggg ttgaatggtg cccgtaactt tcggtagagc ggacggccaa tactcaactt
      361 caaggaatct cacccatgcg cgccggcggg gaaccggagt tcccttcagt gagcgttatt
      421 agttcgccgc tcggtgtgtc gtagatacta gcccctgggg cacttttgaa atttgaataa
      481 gatttatgta atcagtcttt taggtttgac cggttctgcc gcttttttta aaattggatt
      541 tgtaataata aaacgcaatt gtttgttatt gtggcgctct atcatagatg tcgctataaa
      601 cctattcagc acaatatatt gttttcattt taatattgta catataagta gtagggtaca
      661 atcagtaaat tgaacggaga atattattca taaaaatacg atagtaacgg gtgatatatt
      721 cattagaatg aaccgaaacc ggcggtaagg atctgagcta cacatgctca ggttttttac
      781 aacgtgcaca acagaattga aagcaaatat catgcgatca taggcgtctc gcatatctca
      841 ttaaagcagg gatcccgcat cagatctatc aaacacatag atttacgaag atctctaaca
      901 ttaaatcatc agagagataa gtttagggag caagctcttt cgcttac
//
