LOCUS       sequence-73039-e        1148 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Pastrana DV, Tolstov YL, Becker JC, Moore PS, Chang Y, Buck CB
  TITLE     Quantitation of human seroresponsiveness to Merkel cell 
            polyomavirus.
  JOURNAL   PLoS Pathog. 2009 Sep . 5(9):e1000578.
  PUBMED    19750217
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog. 2009 Sep . 5(9):e1000578."
COMMENT     Sequence Label: pGwf
FEATURES             Location/Qualifiers
     source          1..1148
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(106..112,113..130,131..136))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(161..285)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          318..521
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(341..361)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
       61 ccttagctcc tgaaaatctc gacggatcct aactcaaaat ccacacatta tacgagccgg
      121 aagcataaag tgtaaagcct ggggtgccta atgcggccgc catagtgact ggatatgttg
      181 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
      241 tatatcattt tacgtttctc gttcagcttt tttgtacaaa cttgtttgat caagctctag
      301 agaattcctc acgacacctg aaatggaaga aaaaaacttt gaaccactgt ctgaggcttg
      361 agaatgaacc aagatccaaa ctcaaaaagg gcaaattcca aggagaatta catcaagtgc
      421 caagctggcc taacttcagt ctccacccac tcagtgtggg gaaactccat cgcataaaac
      481 ccctcccccc aacctaaaga cgacgtactc caaaagctcg agaactaatc gaggtgcctg
      541 gacggcgccc ggtactccgt ggagtcacat gaagcgacgg ctgaggacgg aaaggccctt
      601 ttcctttgtg tgggtgactc acccgcccgc tctcccgagc gccgcgtcct ccattttgag
      661 ctccctgcag cagggccggg aagcggccat ctttccgctc acgcaactgg tgccgaccgg
      721 gccagccttg ccgcccaggg cggggcgata cacggcggcg cgaggccagg caccagagca
      781 ggccggccag cttgagacta cccccgtccg attctcggtg gccgcgctcg caggccccgc
      841 ctcgccgaac atgtgcgctg ggacgcacgg gccccgtcgc cgcccgcggc cccaaaaacc
      901 gaaataccag tgtgcagatc ttggcccgca tttacaagac tatcttgcca gaaaaaaagc
      961 gtcgcagcag gtcatcaaaa attttaaatg gctagagact tatcgaaagc agcgagacag
     1021 gcgcgaaggt gccaccagat tcgcacgcgg cggccccagc gcccaggcca ggcctcaact
     1081 caagcacgag gcgaaggggc tccttaagcg caaggcctcg aactctccca cccacttcca
     1141 acccgaag
//
