LOCUS       sequence-73042-e        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   de Felippes FF, Wang JW, Weigel D
  TITLE     MIGS: MiRNA Induced Gene Silencing.
  JOURNAL   Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x.
  PUBMED    22211571
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x."
COMMENT     Sequence Label: MIGS3.1 (FF537)
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(105..111,112..129,130..135))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(160..284)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(309..654)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(346..366)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
       61 cttagctcct gaaaatctcg acggctccta actcaaaatc cacacattat acgagccgga
      121 agcataaagt gtaaagcctg gggtgcctaa tggggccgcc aatatgactg gatatgttgt
      181 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
      241 atatcatttt acgtttctcg ttcagctttt ttgtacaaac ttgtgatcct ctagaagctc
      301 gtcccccgtg ttctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa
      361 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
      421 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
      481 tctttgggac cactgtcggc agaggcatct tcaacgatgg cctttccttt atcgcaatga
      541 tggcatttgt aggagccacc ttccttttcc actatcttca caataaagtg acagatagct
      601 gggcaatgga atccgaggag gtttccggat attacccttt gttgaaaagt ctcaattgcc
      661 ctttggtctt ctgagactgt atctttgata tttttggagt agacaagtgt gtcgtgctcc
      721 accatgttga cgaagatttt cttcttgtca ttgagtcgta agagactctg tatgaactgt
      781 tcgccagtct ttacggcgag ttctgttagg tcctctattt gaatctttga ctccatggcc
      841 tttgattcag tgggaactac ctttttagag actccaatct ctattacttg ccttggtttg
      901 tgaagcaagc cttgaatcgt ccatactgga atagtacttc tgatcttgag aaatatatct
      961 ttctctgtgt tcttgatgca gttagtcctg aatcttttga ctgcatcttt aaccttcttg
     1021 ggaaggtatt tgatttcctg gagattattg ctcgggtaga tcgtcttgat gagacctgct
     1081 gcgtaagcct ctctaaccat
//
