LOCUS       sequence-73047-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses V591A and S602D mutated Ataxin1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Kim E, Lu HC, Zoghbi HY, Song JJ
  TITLE     Structural basis of protein complex formation and reconfiguration by
            polyglutamine disease protein Ataxin-1 and Capicua.
  JOURNAL   Genes Dev. 2013 Mar 15;27(6):590-5. doi: 10.1101/gad.212068.112.
  PUBMED    23512657
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.212068"; journalName: "Genes Dev"; date: "2013-03-15- 
            15"; volume: "27"; issue: "6"; pages: "590-5"
COMMENT     Sequence Label: Flag-ATXN1_V591A_S602D
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             206..229
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag
       61 ggagacccaa gcttggtacc gagctcggat ccactagtaa cggccgccag tgtgctggaa
      121 ttccgtgact ttccgtttat cagactaaaa tcagagccat ccagacagtg aaacagtcac
      181 cgtggagggg ggacggcgaa aaatggacta caaagacgac gacgacaaaa aatccaacca
      241 agagcggagc aacgaatgcc tgcctcccaa gaagcgcgag atccccgcca ccagccggtc
      301 ctccgaggag aaggccccta ccctgcccag cgacaaccac cgggtggagg gcacagcatg
      361 gctcccgggc aaccctggtg gccggggcca cgggggcggg aggcatgggc cggcagggac
      421 ctcggtggag cttggtttac aacagggaat aggtttacac aaagcattgt ccacagggct
      481 ggactactcc ccgcccagcg ctcccaggtc tgtccccgtg gccaccacgc tgcctgccgc
      541 gtacgccacc ccgcagccag ggaccccggt gtcccccgta cagtacgctc acctgccgca
      601 caccttccag ttcattgggt cctcccaata cagtggaacc tatgccagct tcatcccatc
      661 acagctgatc cccccaaccg ccaaccccgt caccagtgca gtggcctcgg ccgcaggggc
      721 caccactcca tcccagcgct cccagctgga ggcctattcc actctgctgg ccaacatggg
      781 cagtctgagc cagacgccgg gacacaaggc tgagcagcag cacctcagca gggctccggg
      841 gct
//
