LOCUS       sequence-73057-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Kaper T, Lager I, Looger LL, Chermak D, Frommer WB
  TITLE     Fluorescence Resonance Energy Transfer sensors for quantitative 
            monitoring of pentose and disaccharide accumulation in bacteria.
  JOURNAL   Biotechnol Biofuels. 2008 Jun 3. 1(1):11.
  PUBMED    18522753
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Biofuels. 2008 Jun 3. 1(1):11."
COMMENT     Sequence Label: pGWF1
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..93
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(97..105,106..120)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             join(133..135,136..138,139..651)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(178..199)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(439..458)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     786..807
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgtttacttt aagaaggaga tatacatatg cggggttctc atcatcatca tcatcatggt
       61 atggctagca tgactggtgg acagcaaatg ggtcgggatc tgtacgacga tgacgataag
      121 gatccgggcc gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      181 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      241 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      301 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc
      361 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      421 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      481 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      541 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac
      601 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc
      661 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      721 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
      781 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactgatat cacaagtttg
      841 tacaaaaaag cttgaaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga
      901 ttttgcataa aaaaca
//
