LOCUS       sequence-7307-ex         321 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid contains Top7 K39E K40E V46A and K55E. Please note this is 
            DIFFERENT than what the plasmid name implies..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 321)
  TITLE     Top7 plasmids
REFERENCE   2  (bases 1 to 321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Top7 K39E K40E V48V K55E
FEATURES             Location/Qualifiers
     source          1..321
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             301..318
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggggata tccaggttca agtgaacatt gacgataatg gtaaaaactt cgattatact
       61 tataccgtga ccacagaatc ggaattacag aaggttttga acgaactgaa agactacatc
      121 gaagaacagg gggctaaacg cgcgcgcatc tcgattaccg ctcgtacgga aaaagaagca
      181 gaaaaatttg ccgcgatttt gattaaagtt ttcgccgaac tgggttataa tgatattaac
      241 gttacgtggg acggcgatac ggtgacagtg gaaggacagt tagagggcgg ttctctcgag
      301 caccaccacc accaccactg a
//
