LOCUS       sequence-73083-e        9483 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pHAGE NFKB-TA-LUC-UBC-dTomato-W.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9483)
  AUTHORS   Wilson AA, Kwok LW, Porter EL, Payne JG, McElroy GS, Ohle SJ, 
            Greenhill SR, Blahna MT, Yamamoto K, Jean JC, Mizgerd JP, Kotton DN
  TITLE     Lentiviral delivery of RNAi for in vivo lineage-specific modulation 
            of gene expression in mouse lung macrophages.
  JOURNAL   Mol Ther. 2013 Apr;21(4):825-33. doi: 10.1038/mt.2013.19. Epub 2013 
            Feb 12.
  PUBMED    23403494
REFERENCE   2  (bases 1 to 9483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/mt.2013.19"; journalName: "Mol Ther"; date: "2013-04"; 
            volume: "21"; issue: "4"; pages: "825-33"
COMMENT     Sequence Label: pHAGE NFKB-TA-LUC-UBC-dTomato-W
FEATURES             Location/Qualifiers
     source          1..9483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2270..2301
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2357..2366
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2363..4015
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2429..2447)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        4022..4420
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     CDS             4429..5859
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    5873..6461
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5926..5946)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6344..6355)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6536..6769
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6888..6906)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6974..7078
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7079..7147,7148..7939)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7297..7316)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8110..8698
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8599..8618
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8852..8869
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9109..9130
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9145..9150,9151..9168,9169..9175)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9183..9199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9188..9210
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9207..9223
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9207..9223
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9273..9292
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtggg
     2221 aatttccggg aatttccggg aatttccggg aatttccaga tctagactct agagggtata
     2281 taatggaagc tcgaattcca gcttggcatt ccggtactgt tggtaaaaag cttggcattc
     2341 cggtactgtt ggtaaagcca ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc
     2401 attctatccg ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata
     2461 cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta
     2521 cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa
     2581 tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt
     2641 gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga
     2701 attgctcaac agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt
     2761 gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga
     2821 ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc
     2881 tcccggtttt aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc
     2941 actgatcatg aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag
     3001 aactgcctgc gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc
     3061 ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact
     3121 cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt
     3181 tctgaggagc cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc
     3241 cttcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc
     3301 ttctggtggc gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct
     3361 gccaggtatc aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc
     3421 cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt
     3481 tgtggatctg gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag
     3541 aggtcctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga
     3601 caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt
     3661 catcgttgac cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga
     3721 attggaatcc atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtcttcc
     3781 cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat
     3841 gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg
     3901 cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag
     3961 aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagatcgccg tgtaaggatc
     4021 cggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct
     4081 gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac gctcaggaca
     4141 gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa ggacatttta
     4201 ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg aacaggcgag
     4261 gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg tgaacgccga
     4321 tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc gggatttggg
     4381 tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg ccgcccatat ggtgagcaag
     4441 ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac
     4501 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     4561 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
     4621 cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag
     4681 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt
     4741 ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag
     4801 atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg
     4861 gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag
     4921 gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc
     4981 aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc
     5041 cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg
     5101 ttcctggggc atggcaccgg cagcaccggc agcggcagct ccggcaccgc ctcctccgag
     5161 gacaacaaca tggccgtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc
     5221 atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     5281 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     5341 tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat
     5401 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     5461 ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag
     5521 gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg
     5581 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc
     5641 caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac
     5701 atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc
     5761 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac
     5821 cacctgttcc tgtacggcat ggacgagctg tacaagtaaa tcgatagatc ctaatcaacc
     5881 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     5941 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     6001 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     6061 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     6121 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     6181 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     6241 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     6301 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     6361 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     6421 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgagatcc tttaagacca
     6481 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     6541 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     6601 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6661 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6721 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     6781 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     6841 cccgggttaa ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg
     6901 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     6961 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     7021 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     7081 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     7141 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     7201 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     7261 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     7321 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     7381 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     7441 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     7501 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     7561 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7621 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7681 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7741 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     7801 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7861 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     7921 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     7981 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     8041 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     8101 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     8161 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     8221 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     8281 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     8341 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     8401 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8461 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8521 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8581 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8641 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8701 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     8761 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8821 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     8881 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagcaagct
     8941 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     9001 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctccccgtg
     9061 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     9121 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     9181 aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta cgaatttcac
     9241 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     9301 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
     9361 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
     9421 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
     9481 agt
//
