LOCUS       sequence-73084-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning E.coli expression vector with T7 promoter and C-terminal 
            3C protease cleavage site and 10x His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: p7XC3H
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..379)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(176..195)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 actttaagaa ggagatatac atatgagttg aagagcgacc tgcagactgg ctgtgtataa
       61 gggagcctga catttatatt ccccagaaca tcaggttaat ggcgtttttg atgtcatttt
      121 cgcggtggct gagatcagcc acttcttccc cgataacgga gaccggcaca ctggccatat
      181 cggtggtcat catgcgccag ctttcatccc cgatatgcac caccgggtaa agttcacggg
      241 agactttatc tgacagcaga cgtgcactgg ccagggggat caccatccgt cgcccgggcg
      301 tgtcaataat atcactctgt acatccacaa acagacgata acggctctct cttttatagg
      361 tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt cagcaaaaga gccgttcatt
      421 tcaataaacc gggcgacctc agccatccct tcctgatttt ccgctttcca gcgttcggca
      481 cgcagacgac gggcttcatt ctgcatggtt gtgcttacca gaccggagat attgacatca
      541 tatatgcctt gagcaactga tagctgtcgc tgtcaactgt cactgtaata cgctgcttca
      601 tagcatacct ctttttgaca tacttcgggt atacatatca gtatatattc ttataccgca
      661 aaaatcagcg cgcaaatacg catactgtta tctggctttt agtaagccgg atccacgcgt
      721 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
      781 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
      841 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
      901 cacgtttaaa tcaaaactgg tgaaa
//
