LOCUS       sequence-73088-e        1130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of flag-tagged human EAF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Takahashi H, Parmely TJ, Sato S, Tomomori-Sato C, Banks CA, Kong SE,
            Szutorisz H, Swanson SK, Martin-Brown S, Washburn MP, Florens L, 
            Seidel CW, Lin C, Smith ER, Shilatifard A, Conaway RC, Conaway JW
  TITLE     Human mediator subunit MED26 functions as a docking site for 
            transcription elongation factors.
  JOURNAL   Cell. 2011 Jul 8;146(1):92-104.
  PUBMED    21729782
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-07-8"; volume: "146(1)"; pages: "92-104"
COMMENT     Sequence Label: pcDNA5-Flag-EAF1
FEATURES             Location/Qualifiers
     source          1..1130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..38
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     primer_bind     complement(949..966)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 catggctgca tgccagacta caggacgatg atgacaagct cgagatgaat gggaccgcaa
       61 acccgctgct ggaccgcgag gaacattgcc tgaggctcgg ggagagcttc gagaagcggc
      121 cgcgggcctc cttccacact attcgttatg attttaaacc agcatctata gacacttcct
      181 gtgaaggaga gcttcaagtt ggcaaaggag atgaagtcac aattacactt ccacatatcc
      241 ctggatccac accacccatg actgtgttca aggggaacaa acggccttac cagaaagact
      301 gtgtgcttat tattaatcat gacactggtg aatttgtgct ggaaaaactc agtagcagca
      361 ttcaggtgaa gaaaacaaga gctgagggca gcagtaaaat ccaggcccga atggaacagc
      421 agcccactcg tcctccacag acgtcacagc caccaccacc tccaccacct atgccattca
      481 gagctccaac gaagcctcca gttggaccca aaacttctcc cttgaaagat aacccctcac
      541 ctgaacctca gttggatgac atcaaaagag agctgagggc tgaagttgac attattgaac
      601 aaatgagcag cagcagtggg agcagctctt cagactctga gagctcttcg ggaagtgatg
      661 acgatagctc cagcagtgga ggcgaggaca atggcccagc ctctcctccg cagccttcac
      721 accagcagcc ctacaacagt aggcctgccg ttgccaatgg aaccagccgg ccacaaggaa
      781 gcaaccagct catgaacgcc ctcagaaatg acttgcagtt gagtgagtct ggcagtgaca
      841 gtgatgacta gagatccact agtccagtgt ggtggaattc tgcagatatc cagcacagtg
      901 gcggccgctc gatcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc
      961 cttctagttg ccagccatct gttgtttgcc cctcccccgt gcttccttga cctggaaggt
     1021 gccactcccc actgtctttc taataaatga gaaatgcatc gcattgtctg agtagtgtca
     1081 ttctattctg gggggtgggg tggggcaaga cagcaggggg agaatggcaa
//
