LOCUS       sequence-73089-e        1130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of flag-tagged human EAF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Takahashi H, Parmely TJ, Sato S, Tomomori-Sato C, Banks CA, Kong SE,
            Szutorisz H, Swanson SK, Martin-Brown S, Washburn MP, Florens L, 
            Seidel CW, Lin C, Smith ER, Shilatifard A, Conaway RC, Conaway JW
  TITLE     Human mediator subunit MED26 functions as a docking site for 
            transcription elongation factors.
  JOURNAL   Cell. 2011 Jul 8;146(1):92-104.
  PUBMED    21729782
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-07-8"; volume: "146(1)"; pages: "92-104"
COMMENT     Sequence Label: pcDNA5-Flag-EAF2
FEATURES             Location/Qualifiers
     source          1..1130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..37
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(924..941)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    930..1039
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 catgctgcat gcagactaca aggacgatga tgacaagctc gagatgaata gcgcagcggg
       61 attctcacac ctagaccgtc gcgagcgggt tctcaagtta ggggagagtt tcgagaagca
      121 gccgcgctgc gccttccaca ctgtgcgcta tgacttcaaa cctgcttcta ttgacacttc
      181 ttctgaagga taccttgagg ttggtgaagg tgaacaggtg accataactc tgccaaatat
      241 agaaggttca actccaccag taactgtttt caaaggttca aaaaaacctt acttaaaaga
      301 atgcattttg attattaacc atgatactgg agaatgtcgg ctagaaaaac tcagcagcaa
      361 catcactgta aaaaaaacaa gagttgaagg aagcagtaaa attcagtatc gtaaagaaca
      421 acagcaacaa caaatgtgga attcagccag gactcccaat cttgtaaaac attctccatc
      481 tgaagataag atgtccccag catctccaat agatgatatc gaaagagaac tgaaggcaga
      541 agctagtcta atggaccaga tgagtagttg tgatagttca tcagattcca aaagttcatc
      601 atcttcaagt agtgaggata gttctagtga ctcagaagat gaagattgca aatcctctac
      661 ttctgataca gggaattgtg tctcaggaca tcctaccatg acacagtaca ggattcctga
      721 tatagatgcc agtcataata gatttcgaga caacagtggc cttctgatga atactttaag
      781 aaatgatttg cagctgagtg aatcacgaag tgacagtgat gactgaggat ccactagtcc
      841 agtgtggtgg aattctgcag atatccagca cagtggcggc cgctcgatcg agtctagagg
      901 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
      961 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttctaa
     1021 taaaatgaga aattgcatcg cattgtctga gtaggtgtca ttctattctg acgctgggtt
     1081 ggacagacag caagggggga ggattgggaa gacaatagca gcatgctggg
//
