LOCUS       sequence-73091-e        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of flag-tagged human Med26-NTD- K74A K75A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Takahashi H, Parmely TJ, Sato S, Tomomori-Sato C, Banks CA, Kong SE,
            Szutorisz H, Swanson SK, Martin-Brown S, Washburn MP, Florens L, 
            Seidel CW, Lin C, Smith ER, Shilatifard A, Conaway RC, Conaway JW
  TITLE     Human mediator subunit MED26 functions as a docking site for 
            transcription elongation factors.
  JOURNAL   Cell. 2011 Jul 8;146(1):92-104.
  PUBMED    21729782
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-07-8"; volume: "146(1)"; pages: "92-104"
COMMENT     Sequence Label: pcDNA5-F-Med26-NTD- K74A K75A
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..43
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(484..501)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    490..714
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(847..866)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccaagcatgc tgcatgccag actacaagga cgatgatgac aagctcgaga tgacagcggc
       61 tccggcgtct ccgcagcaga tcagggaccg gctgctgcag gccatcgacc cccagagcaa
      121 catccggaac atggtggcgg tgctggaagt catctccagc ctggagaaat accctattac
      181 caaagaggca cttgaggaaa cacgacttgg gaagctcatc aacgcgcaag aaaaccaaga
      241 acgaggagct cgccaagcgg gccgcggcgc tgctgcggag ctggcagaag ctcatcgagc
      301 cggcacacca gcatgaggcg gcgctgcggg ggctggcggg ggccaccggc tctgccaacg
      361 ggggcgcaca caactgccgg ccgtgaggat ccactagtcc agtgtggtgg aattctgcag
      421 atatccagca cagtggcggc cgctcgatcg agtctagagg gcccgtttaa acccgctgat
      481 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
      541 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
      601 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
      661 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
      721 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
      781 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      841 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      901 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
      961 ccaaaaactt gattagggtg atggttcacg tacctagaag ttcctattcc gaagttccta
     1021 ttcttctaga aagtatagga cttccttggc caa
//
