LOCUS       sequence-73094-e         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of flag-tagged Med26-NTD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Takahashi H, Parmely TJ, Sato S, Tomomori-Sato C, Banks CA, Kong SE,
            Szutorisz H, Swanson SK, Martin-Brown S, Washburn MP, Florens L, 
            Seidel CW, Lin C, Smith ER, Shilatifard A, Conaway RC, Conaway JW
  TITLE     Human mediator subunit MED26 functions as a docking site for 
            transcription elongation factors.
  JOURNAL   Cell. 2011 Jul 8;146(1):92-104.
  PUBMED    21729782
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-07-8"; volume: "146(1)"; pages: "92-104"
COMMENT     Sequence Label: pcDNA5-F-Med26-NTD
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..36
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     primer_bind     complement(434..451)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    440..551
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 catgctgcat gcagactaca ggacgatgat gacaagctcg acatgacagc ggctccggcg
       61 tctccgcagc agatcaggga ccggctgctg caggccatcg acccccagag caacatccgg
      121 aacatggtgg cggtgctgga agtcatctcc agcctggaga aataccctat taccaaagag
      181 gcacttgagg aaacacgact tgggaagctc atcaacgacg tccgcaagaa aaccaagaac
      241 gaggagctcg ccaagcgggc caagaagctg ctgcggagct ggcagaagct catcgagccg
      301 gcacaccagc atgaggcggc gctgcggggg ctggcggggg ccaccggctc tgccaacggg
      361 ggcgcacaca actgccggcc gtgaggatcc ggtaccgtcg agtctagagg gcccgtttaa
      421 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
      481 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
      541 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcaa
      601 gacagcaatg ttcaattttg ggtgtcaata acattttggt taccgataca tttgactctt
      661 tgcttctaag tagagataag gtttggttct tgtcctgtct ttgccaacg
//
