LOCUS       sequence-73099-e         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Frank S, Skryabin BV, Greber B
  TITLE     A modified TALEN-based system for robust generation of knock-out 
            human pluripotent stem cell lines and disease models.
  JOURNAL   BMC Genomics. 2013 Nov 9;14(1):773.
  PUBMED    24206569
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2013-11-9"; volume: "14(1)"; pages: "773"
COMMENT     Sequence Label: pTAL7a
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     91..113
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     105..122
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     106..122
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(198..214)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(198..214)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(211..233)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    222..238
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(246..252,253..270,271..276))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    291..312
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
       61 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      121 gtgagcgcgc ggcgaattgg gtaacgggcc acccctcgag gtcctccagc ttttgttgcg
      181 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
      241 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
      301 taactcacat taattgcgtt gcgctcactg cccgctttcc accggtcgtc tccaacgatc
      361 atctggtcgc tctcgcttgc ctcggtggac ggcccgctct ggacgcagtc aaaaagggtc
      421 tcccccatgc tcccgcactg atcaagagaa ccaacaggag aattcctgag ggatccgatc
      481 gtttaaacca gctcgtgaaa agcgaactcg aagaaaagaa aagtgaactg cggcacaaac
      541 tgaaatacgt cccacatgaa tacattgagc tgatcgagat tgctaggaac tccacccagg
      601 acagaatcct cgagatgaaa gtgatggaat tctttatgaa agtctacggg tatcggggca
      661 agcacctggg cggatctcgc aaaccagatg gggcaatcta cactgtgggt agtcccatcg
      721 actatggcgt gattgtcgat accaaggcct acagtggggg ttataatctg cccattggac
      781 aggctgacga gatgcagcga tacgtggagg aaaaccagac aagaaataag catatcaacc
      841 ccaatgagtg gtggaaagtg tatcctagct ccgtcactga attcaagttt ctcttcgtgt
      901 caggccactt taagggaaac tacaaagcac agctgaccag gctcaatcat attacaaact
      961 gcaa
//
