LOCUS       sequence-73106-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ads under Pgrac promoter (inducible by IPTG) in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Zhou K, Zou R, Zhang C, Stephanopoulos G, Too HP
  TITLE     Optimization of amorphadiene synthesis in Bacillus subtilis via 
            transcriptional, translational, and media modulation.
  JOURNAL   Biotechnol Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900.
  PUBMED    23483530
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900."
COMMENT     Sequence Label: pHT-ads
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(162..179)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gactattcct aataagccga tattagcctc gtatgtttca accatttgtt ccaggctaag
       61 gtataaactt ttcagttgca gacaaagatc tccatggacg cgtgacgtga aaaaagcccg
      121 ctcattaggc gggctgcccc ggggacgtcg actctagatc agtgatggtg atgatgatgg
      181 atggacattg ggtaaaccag cagggacttg atcaggtgtt tgtattcgtc acccatacgg
      241 gtgaagttat ctttgcccgc atactgcact tccagaaact ggcacagata aatcactgcc
      301 atcagcagcg ggcgtgggat gtttttagta gtcaggtact cacggttgat gtctttccat
      361 acatcctcaa cctctttgta gatcagggtc tgcgcatact cttcgttaac gttgtattct
      421 ttcatgtaag attccagaga gctgctggag tgtttacgtt cctgctcggc tttatgggtc
      481 atcagatcgt tcaggcgacg gcccaggata ccagagtaac ggaacagcgg aggcgcagac
      541 actgcccatt caacgctttc tttcgtaaaa atgtcggaca tacccaggta gcaagtggta
      601 gtcagcaggt ttgcgccacc ggtgatgata actaccgggt cgtgttcttc ggtggtcggg
      661 atgtgaccct cgtttgccca cttcgcctcc accatcaggt tgcggacgaa ttctttcaca
      721 aactccttgc cacagttgaa cagatcagtg cgaccttctt ttgccaggaa ctcttccatt
      781 tcggtataag tatccatgaa cagtttatag atcggtttca tgtattccgg cagggtatcc
      841 aggcaagtga tagaccagcg ttcaacagcc tcggta
//
