LOCUS       sequence-73107-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ads under Pgrac promoter (inducible by IPTG) in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Zhou K, Zou R, Zhang C, Stephanopoulos G, Too HP
  TITLE     Optimization of amorphadiene synthesis in Bacillus subtilis via 
            transcriptional, translational, and media modulation.
  JOURNAL   Biotechnol Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900.
  PUBMED    23483530
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900."
COMMENT     Sequence Label: pHT-ads
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    364..388
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tgaatatttg attgatcgta accagatgaa gcactctttc cactatccct acagtgttat
       61 ggcttgaaca atcacgaaac aataattggt acgtacgatc tttcagccga ctcaaacatc
      121 aaatcttaca aatgtagtct ttgaaagtat tacatatgta agatttaaat gcaaccgttt
      181 tttcggaagg aaatgatgac ctcgtttcca ccggaattag cttggtacca gctattgtaa
      241 cataatcggt acgggggtga aaaagctaac ggaaaaggga gcggaaaaga atgatgtaag
      301 cgtgaaaaat tttttatctt atcacttgaa attggaaggg agattcttta ttataagaat
      361 tgtggaattg tgagcggata acaattccca attaaaggag gaaggatcca tgtctctgac
      421 tgaggaaaaa ccaatccgtc cgatcgcaaa ctttccgccg agcatctggg gtgatcagtt
      481 cctgatctac cagaagcagg tcgagcaagg cgtagaacag atcgtgaacg acctgaaaaa
      541 ggaggtacgt cagctgctga aagaggctct ggacatcccg atgaaacatg cgaacctgct
      601 gaagctgatt gatgagattc agcgcctggg tatcccatac cacttcgaac gtgaaattga
      661 tcacgcgctg cagtgtattt atgaaacgta tggtgataac tggaacggtg accgtagctc
      721 cctgtggttc cgtctgatgc gtaagcaggg ttattacgtg acctgcgacg tcttcaacaa
      781 ctacaaagac aagaacggcg cgtttaaaca gagcctggcg aatgacgttg aaggcctgct
      841 ggagctgtat gaagcaacct ctatgcgtgt tccg
//
