LOCUS       sequence-73124-e         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human codon optimized Cas9 nuclease from Streptococcus pyogenes 
            (Cbh-3X-FLAG-NLS-SpCas9-NLS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F
  TITLE     Genome engineering using the CRISPR-Cas9 system.
  JOURNAL   Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. 
            Epub 2013 Oct 24.
  PUBMED    24157548
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2013.143"; journalName: "Nat Protoc"; date: 
            "2013-11"; volume: "8"; issue: "11"; pages: "2281-308"
COMMENT     Sequence Label: pSpCas9 (PX165)
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      56..65
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             65..130
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             137..157
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgtttaatta cctggagcac ctgcctgaaa tcactttttt tcaggttgga ccggtgccac
       61 catggactat aaggaccacg acggagacta caaggatcat gatattgatt acaaagacga
      121 tgacgataag atggccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc
      181 cgacaagaag tacagcatcg gcctggacat cggcaccaac tctgtgggct gggccgtgat
      241 caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca
      301 cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc
      361 cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta
      421 tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct tccacagact
      481 ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa
      541 catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa
      601 actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat
      661 gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt
      721 ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat
      781 caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg
      841 gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct
      901 gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga
      961 tgccaaactg c
//
