LOCUS       sequence-73131-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning E.coli expression vector with araBAD promoter and 
            N-terminal 10x His tag and 3C protease cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pBXNH3
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             63..92
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             93..116
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(165..470)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(267..286)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 atcgcaactc tctactgttt ctccataccc gttttttggg ctaacaggag gaattaacca
       61 tgcaccatca tcatcaccat catcatcatc atttagaagt tttgtttcaa ggtccaagta
      121 gaagagcgag ctgcagactg gctgtgtata agggagcctg acatttatat tccccagaac
      181 atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc
      241 ccgataacgg agaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc
      301 ccgatatgca ccaccgggta aagttcacgg gagactttat ctgacagcag acgtgcactg
      361 gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca
      421 aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagcc
      481 cctgttctcg tcagcaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc
      541 ttcctgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt
      601 tgtgcttacc agaccggaga tattgacatc atatatgcct tgagcaactg atagctgtcg
      661 ctgtcaactg tcactgtaat acgctgcttc atagcatacc tctttttgac atacttcggg
      721 tatacatatc agtatatatt cttataccgc aaaaatcagc gcgcaaatac gcatactgtt
      781 atctggcttt tagtaagccg gatccacgcg tttacgcccc gccctgccac tcatcgcagt
      841 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
      901 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg ccca
//
