LOCUS       sequence-73133-e         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning E.coli expression vector with araBAD promoter and 
            C-terminal 3C protease cleavage site and 10x His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pBXC3H
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(111..416)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(213..232)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 atcgcaactc tctactgttt ctccataccc gttttttggg ctaacaggag gaattaacca
       61 tgagttgaag agcgacctgc agactggctg tgtataaggg agcctgacat ttatattccc
      121 cagaacatca ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact
      181 tcttccccga taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt
      241 tcatccccga tatgcaccac cgggtaaagt tcacgggaga ctttatctga cagcagacgt
      301 gcactggcca gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca
      361 tccacaaaca gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca
      421 ccagcccctg ttctcgtcag caaaagagcc gttcatttca ataaaccggg cgacctcagc
      481 catcccttcc tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg
      541 catggttgtg cttaccagac cggagatatt gacatcatat atgccttgag caactgatag
      601 ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag catacctctt tttgacatac
      661 ttcgggtata catatcagta tatattctta taccgcaaaa atcagcgcgc aaatacgcat
      721 actgttatct ggcttttagt aagccggatc cacgcgttta cgccccgccc tgccactcat
      781 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
      841 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
      901 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
      961 aactcaccca gggattggct gagacgaaa
//
