LOCUS       sequence-73135-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning E.coli expression vector with araBAD promoter, N-terminal
            10x His tag, 3C protease cleavage site and C-terminal Avi-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pBXNH3CA
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..91
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             92..115
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(164..469)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(266..285)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg ttttttgggc taacaggagg aattaaccat
       61 gcaccatcat catcaccatc atcatcatca tttagaagtt ttgtttcaag gtccaagtag
      121 aagagcgagc tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca
      181 tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc
      241 cgataacgga gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc
      301 cgatatgcac caccgggtaa agttcacggg agactttatc tgacagcaga cgtgcactgg
      361 ccagggggat caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa
      421 acagacgata acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagccc
      481 ctgttctcgt cagcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct
      541 tcctgatttt ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt
      601 gtgcttacca gaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc
      661 tgtcaactgt cactgtaata cgctgcttca tagcatacct ctttttgaca tacttcgggt
      721 atacatatca gtatatattc ttataccgca aaaatcagcg cgcaaatacg catactgtta
      781 tctggctttt agtaagccgg atccacgcgt ttacgccccg ccctgccact catcgcagta
      841 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
      901 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttg
//
