LOCUS       sequence-73136-e         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning E.coli expression vector with araBAD promoter and 
            C-terminal 3C protease cleavage site, GFP and 10x His tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pBXC3GH
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(90..96,97..114,115..120))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(145..269)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(288..308)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(312..332)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             359..382
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(416..444)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
       61 tctcgacgga tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa
      121 gcctggggtg cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat
      181 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
      241 tctcgttcag cttttttgta caaacttgtg atatcgaatt cgctagctac ctttctcttc
      301 ttttttggat ctacctttct cttctttttt ggggatccac tagttcagct cttctgcatt
      361 agaagttttg tttcaaggtc cacaattcag caaaggagaa gaacttttca ctggagttgt
      421 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga
      481 gggtgaaggt gatgctacat acggaaaact cacccttaaa tttatttgca ctactggaaa
      541 actacctgtt ccttggccaa cacttgtcac tactctgacc tatggtgttc aatgcttttc
      601 ccgttatccg gatcacatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta
      661 tgtacaggaa cgcactatat ctttcaaaga tgacgggaac tacaagacgc gtgctgaagt
      721 caagtttgaa ggtgataccc ttgttaatcg tatcgagtta aagggtattg attttaaaga
      781 agatggaaac attctcggac acaaactaga gtacaactat aactcacaca atgtatacat
      841 cacggcagac aaacaaaaga atggaatcaa agctaacttc aaaattcgcc acaacattga
      901 agatggttcc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
      961 tgtcctt
//
