LOCUS       sequence-73137-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning E.coli expression vector with araBAD promoter and 
            C-terminal 3C protease cleavage site, GFP and 10x His tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pBXC3GH
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(110..415)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(212..231)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg ttttttgggc taacaggagg aattaaccat
       61 gagttgaaga gcgacctgca gactggctgt gtataaggga gcctgacatt tatattcccc
      121 agaacatcag gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt
      181 cttccccgat aacggagacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt
      241 catccccgat atgcaccacc gggtaaagtt cacgggagac tttatctgac agcagacgtg
      301 cactggccag ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat
      361 ccacaaacag acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac
      421 cagcccctgt tctcgtcagc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc
      481 atcccttcct gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc
      541 atggttgtgc ttaccagacc ggagatattg acatcatata tgccttgagc aactgatagc
      601 tgtcgctgtc aactgtcact gtaatacgct gcttcatagc atacctcttt ttgacatact
      661 tcgggtatac atatcagtat atattcttat accgcaaaaa tcagcgcgca aatacgcata
      721 ctgttatctg gcttttagta agccggatcc acgcgtttac gccccgccct gccactcatc
      781 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
      841 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
      901 ggtgaaaacg ggg
//
