LOCUS       sequence-73142-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning E.coli expression vector with araBAD promoter, N-terminal
            Avi-tag, C-terminal 3C protease cleavage site, GFP and 10x His tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pBXNAC3GH
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(88..94,95..112,113..118))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(143..267)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(286..306)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(310..330)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             357..380
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(414..442)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
       61 tcgacggatc ctaactcaaa atccacacat tatacgagcc ggaagcataa agtgtaaagc
      121 ctggggtgcc taatgcggcc gccatagtga ctggatatgt tgtgttttac agtattatgt
      181 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
      241 tcgttcagct tttttgtaca aacttgtgat atcgaattcg ctagctacct ttctcttctt
      301 ttttggatct acctttctct tcttttttgg ggatccacta gttcagctct tctgcattag
      361 aagttttgtt tcaaggtcca caattcagca aaggagaaga acttttcact ggagttgtcc
      421 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
      481 gtgaaggtga tgctacatac ggaaaactca cccttaaatt tatttgcact actggaaaac
      541 tacctgttcc ttggccaaca cttgtcacta ctctgaccta tggtgttcaa tgcttttccc
      601 gttatccgga tcacatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg
      661 tacaggaacg cactatatct ttcaaagatg acgggaacta caagacgcgt gctgaagtca
      721 agtttgaagg tgataccctt gttaatcgta tcgagttaaa gggtattgat tttaaagaag
      781 atggaaacat tctcggacac aaactagagt acaactataa ctcacacaat gtatacatca
      841 cggcagacaa acaaaagaat ggaatcaaag ctaacttcaa aattcgccac aacattgaag
      901 atggttccgt tcaactagca gaccattatc aacaaaatac tccaat
//
