LOCUS       sequence-73144-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning E.coli expression vector with araBAD promoter, N-terminal
            Avi-tag, C-terminal 3C protease cleavage site, GFP and 10x His tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pBXNAC3GH
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             65..109
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(158..463)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(260..279)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg ttttttgggc taacaggagg aattaaccat
       61 ggctggtctg aacgatatct tcgaagctca gaaaatcgaa tggcacgaaa gttgaagagc
      121 gacctgcaga ctggctgtgt ataagggagc ctgacattta tattccccag aacatcaggt
      181 taatggcgtt tttgatgtca ttttcgcggt ggctgagatc agccacttct tccccgataa
      241 cggagaccgg cacactggcc atatcggtgg tcatcatgcg ccagctttca tccccgatat
      301 gcaccaccgg gtaaagttca cgggagactt tatctgacag cagacgtgca ctggccaggg
      361 ggatcaccat ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc acaaacagac
      421 gataacggct ctctctttta taggtgtaaa ccttaaactg catttcacca gcccctgttc
      481 tcgtcagcaa aagagccgtt catttcaata aaccgggcga cctcagccat cccttcctga
      541 ttttccgctt tccagcgttc ggcacgcaga cgacgggctt cattctgcat ggttgtgctt
      601 accagaccgg agatattgac atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa
      661 ctgtcactgt aatacgctgc ttcatagcat acctcttttt gacatacttc gggtatacat
      721 atcagtatat attcttatac cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc
      781 ttttagtaag ccggatccac gcgtttacgc cccgccctgc cactcatcgc agtactgttg
      841 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat
      901 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaa
//
