LOCUS       sequence-73148-e        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Pastrana DV, Tolstov YL, Becker JC, Moore PS, Chang Y, Buck CB
  TITLE     Quantitation of human seroresponsiveness to Merkel cell 
            polyomavirus.
  JOURNAL   PLoS Pathog. 2009 Sep . 5(9):e1000578.
  PUBMED    19750217
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog. 2009 Sep . 5(9):e1000578."
COMMENT     Sequence Label: pGwf
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    106..230
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(271..576)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(373..392)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ataccagtca atctttcaca aattttgtaa tccagaggtt gattatcgat aagcttgata
       61 tcgaattcct gcagcccggg ggatccacta gccattctag atcgaaccac tttgtacaag
      121 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
      181 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg gtcgacctgc
      241 agactggctg tgtataaggg agcctgacat ttatattccc cagaacatca ggttaatggc
      301 gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga taacggagac
      361 cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga tatgcaccac
      421 cgggtaaagt tcacggggga ctttatctga cagcagacgt gcactggcca gggggatcac
      481 catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca gacgataacg
      541 gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagcccctg ttctcgtcag
      601 caaaagagcc gttcatttca ataaaccggg cgacctcagc catcccttcc tgattttccg
      661 ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgtg cttaccagac
      721 cggagatatt gacatcatat atgccttgag caactgatag ctgtcgctgt caactgtcac
      781 tgtaatacgc tgcttcatag catacctctt tttgacatac ttcgggtata catatcagta
      841 tatattctta taccgcaaaa atcagcgcgc aaatacgcat actgttatct ggcttttagt
      901 aagccggatc ctctagatta cgccccgcct gccactcatc gcagtactgt tgtaattcat
      961 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgcca
//
