LOCUS       sequence-73149-e         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning E.coli expression vector with araBAD promoter and 
            N-terminal 10x His, 3C protease cleavage site and Avi-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pBXNH3A
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..91
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             92..115
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             116..160
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(209..514)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(311..330)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg ttttttgggc taacaggagg aattaaccat
       61 gcaccatcat catcaccatc atcatcatca tttagaagtt ttgtttcaag gtccaggtct
      121 gaacgatatc ttcgaagctc agaaaatcga atggcacgaa agttgaagag cgacctgcag
      181 actggctgtg tataagggag cctgacattt atattcccca gaacatcagg ttaatggcgt
      241 ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg
      301 gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg
      361 ggtaaagttc acgggagact ttatctgaca gcagacgtgc actggccagg gggatcacca
      421 tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc
      481 tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcagca
      541 aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct
      601 ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct taccagaccg
      661 gagatattga catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg
      721 taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca tatcagtata
      781 tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg cttttagtaa
      841 gccggatcca cgcgtttacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
      901 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
      961 catcagcacc ttgtcgcct
//
