LOCUS       sequence-73155-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning Lactococcus lactis expression vector (VBEx) with nisin 
            promoter and C-terminal 3C protease cleavage site and 10x His tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pREXC3H
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
       61 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgct cttcaactca
      121 tggtgagtgc ctccttataa tttattttgt agttccttcg aacgaaatca ttgtatctaa
      181 caaacttcag aatttaatca gagccgttta ttatgctcgc gttatcgaca ataatattat
      241 taccaatact ttctcaagat agaattaaga ctgttttaga tttgttaatg tttctattgt
      301 cagtatagtt ataagactag atctggagct gtaatataaa aaccttcttc aactaacggg
      361 gcaggttagt gacattagaa aaccgactgt aaaaagtaca gtcggcatta tctcatatta
      421 taaaagccag tcattaggcc tatctgacaa ttcctgaata gagttcataa acaatccagc
      481 atgataacca tcacaaacag aatgatgtac ctgtaaagat agcggtaaat atattgaatt
      541 acctttatta atgaattttc ctgctgtaat aatgggtaga aggtaattac tattattatt
      601 gatatttaag ttaaacccag taaatgaagt ccaaggaata atagaaagag aaaaagcatt
      661 ttcaggtata ggtgttttgg gaaacaattt ccccgaacca ttatatttct ctacatcaga
      721 aaggtataaa tcataaaact ctttgaagtc attctttaca ggagtccaaa taccagagaa
      781 tgttttagat acaccatcaa aaattgtata aagtggctct aacttatccc aataacctaa
      841 ctctccgtcg ctattgtaac cagttctaaa agctgtattt gagtttatca cccttgtcac
      901 taagaaaata aatgcagggt aaaattta
//
