LOCUS       sequence-73156-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Kowalska E, Ripperger JA, Muheim C, Maier B, Kurihara Y, Fox AH, 
            Kramer A, Brown SA
  TITLE     Distinct roles of DBHS family members in the circadian 
            transcriptional feedback loop.
  JOURNAL   Mol Cell Biol. 2012 Nov;32(22):4585-94. doi: 10.1128/MCB.00334-12. 
            Epub 2012 Sep 10.
  PUBMED    22966205
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00334-12"; journalName: "Mol Cell Biol"; date: 
            "2012-11"; volume: "32"; issue: "22"; pages: "4585-94"
COMMENT     Sequence Label: pSCT-GAL93-DEST
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(107..231)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             257..298
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             308..325
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
       61 atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat
      121 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
      181 gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg ttgatctaga
      241 gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg
      301 taccggtcat catcaccatc accattgagt ttaaacccgc tgatcctgcg tcgaccccgg
      361 ggatctgggc ccccccgacc gatgtcagcc tgggggacga gctccactta gacggcgagg
      421 acgtggcgat ggcgcatgcc gacgcgctag acgatttcga tctggacatg ttgggggacg
      481 gggattcccc gggtctagtt agtcagtcta gactagctga ctagagctga gaacttcagg
      541 gtgagtttgg ggacccttga ttgttctttc tttttcgcta ttgtaaaatt catgttatat
      601 ggagggggca aagttttcag ggtgttgttt agaatgggaa gatgtccctt gtatcaccat
      661 ggaccctcat gataattttg tttctttcac tttctactct gttgacaacc attgtctcct
      721 cttattttct tttcattttc tgtaactttt tcgttaaact ttagcttgca tttgtaacga
      781 atttttaaat tcacttttgt ttatttgtca gattgtaagt actttctcta atcacttttt
      841 tttcaaggca atcagggtat attatattgt acttcagcac agttttagag aacaattgtt
      901 ataattaaat
//
