LOCUS       sequence-73158-e         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning Lactococcus lactis expression vector (VBEx) with nisin 
            promoter and N-terminal 10x His tag and 3C protease cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pREXNH3
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(118..141)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(142..171)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
       61 ggaatataaa tgtcaggctc ccttatacac agccagtctg cagctcgctc ttctacttgg
      121 accttgaaac aaaacttcta aatgatgatg atgatggtga tgatgatggt gcatggtgag
      181 tgcctcctta taatttattt tgtagttcct tcgaacgaaa tcattgtatc taacaaactt
      241 cagaatttaa tcagagccgt ttattatgct cgcgttatcg acaataatat tattaccaat
      301 actttctcaa gatagaatta agactgtttt agatttgtta atgtttctat tgtcagtata
      361 gttataagac tagatctgga gctgtaatat aaaaaccttc ttcaactaac ggggcaggtt
      421 agtgacatta gaaaaccgac tgtaaaaagt acagtcggca ttatctcata ttataaaagc
      481 cagtcattag gcctatctga caattcctga atagagttca taaacaatcc agcatgataa
      541 ccatcacaaa cagaatgatg tacctgtaaa gatagcggta aatatattga attaccttta
      601 ttaatgaatt ttcctgctgt aataatgggt agaaggtaat tactattatt attgatattt
      661 aagttaaacc cagtaaatga agtccaagga ataatagaaa gagaaaaagc attttcaggt
      721 ataggtgttt tgggaaacaa tttccccgaa ccattatatt tctctacatc agaaaggtat
      781 aaatcataaa actctttgaa gtcattcttt acaggagtcc aaataccaga gaatgtttta
      841 gatacaccat caaaaattgt ataaagtggc tctaacttat cccaataacc taactctccg
      901 tcgctattgt aaccagttct aaaagctgta tttgagttta tcacccttgt cactaagaaa
      961 ataaatgcag ggtaaaattt a
//
