LOCUS       sequence-73160-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning E.coli expression vector with T7 promoter and C-terminal 
            3C protease cleavage site and 10x His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: p7XC3H
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(71..100)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(101..124)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             151..171
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             175..195
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    214..338
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(363..368,369..386,387..393)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(513..532)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagct tctgagatga gtttttgttc
       61 tagattatta atgatgatga tgatggtgat gatgatggtg tggaccttga aacaaaactt
      121 ctaatgcaga agagctgaac tagtggatcc ccaaaaaaga agagaaaggt agatccaaaa
      181 aagaagagaa aggtagctag cgaattcgat atcacaagtt tgtacaaaaa agctgaacga
      241 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
      301 acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag
      361 gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga
      421 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
      481 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
      541 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
      601 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
      661 aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
      721 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
      781 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
      841 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
      901 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcct
//
