LOCUS       sequence-73163-e         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX sequencing vector for subcloning, kanamycin marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pINIT_kan
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(170..256)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
       61 gaatataaat gtcaggctcc cttatacaca gccagtctgc agtcgactag aagagcggcc
      121 aaccaggccg tgcagcgctt gcggccggcc gctaggtcta gggcggcgga tttgtcctac
      181 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
      241 cctttcgttt tatttgatgc ctctagacga cccaccgccg agaccgcggc cgcaatgcca
      301 ataggatatc ggcattttct tttgcgtttt tatttgttaa ctgttaattg tccttgttca
      361 aggatgctgt ctttgacaac agatgttttc ttgcctttga tgttcagcag gaagctaggc
      421 gcaaacgttg attgtttgtc tgcgtagaat cctctgtttg tcatatagct tgtaatcacg
      481 acattgtttc ctttcgcttg aggtacagcg aagtgtgagt aagtaaaggt tacatcgtta
      541 ggatcaagat ccatttttaa cacaaggcca gttttgttca gcggcttgta tgggccagtt
      601 aaagaattag aaacataacc aagcatgtaa atatcgttag acgtaatgcc gtcaatcgtc
      661 atttttgatc cgcgggagtc agtgaacagg taccatttgc cgttcatttt aaagacgttc
      721 gcgcgttcaa tttcatctgt tactgtgtta gatgcaatca gcggtttcat cacttttttc
      781 agtgtgtaat catcgtttag ctcaatcata ccgagagcgc cgtttgctaa ctcagccgtg
      841 cgttttttat cgctttgcag aagtttttga ctttcttgac ggaagaatga tgtgcttttg
      901 ccatagtatg ctttgttaaa taaagattct tcgccttggt agccatcttc agttccagtg
      961 tttgcttcaa atactaagta tttgtggcct ttatct
//
