LOCUS       sequence-73164-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning E.coli expression vector with T7 promoter and N-terminal 
            10x His tag and 3C protease cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: p7XNH3
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..31)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..93
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             97..117
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    136..260
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(285..290,291..308,309..315)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(435..454)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cagccggatc tcagtggtgg tggtggtggt gctcgagtag ttattatgca gaagagctga
       61 actagtggat ccccaaaaaa gaagagaaag gtagatccaa aaaagaagag aaaggtagct
      121 agcgaattcg atatcacaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata
      181 aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca
      241 caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca ctttatgctt
      301 ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg
      361 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
      421 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
      481 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
      541 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga
      601 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
      661 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
      721 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
      781 ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt
      841 taaacgtggc caatatggac aacttcttcg cccc
//
