LOCUS       sequence-73167-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning Saccharomyces cerevisiae expression vector with GAL1 
            promoter and N-terminal 10x His tag and 3C protease cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pYEXNH3
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(119..142)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(143..172)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     primer_bind     complement(201..220)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(202..220)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(252..693)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(258..281)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    576..693
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(802..821)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
       61 gggaatataa atgtcaggct cccttataca cagccagtct gcagctcgct cttctacttg
      121 gaccttgaaa caaaacttct aaatgatgat gatgatggtg atgatgatgg tgcatggtta
      181 atggtaccaa gcttaatatt ccctatagtg agtcgtatta cagctgctag tagtccgatc
      241 cggggttttt tctccttgac gttaaagtat agaggtatat taacaatttt ttgttgatac
      301 ttttattaca tttgaataag aagtaataca aaccgaaaat gttgaaagta ttagttaaag
      361 tggttatgca gtttttgcat ttatatatct gttaatagat caaaaatcat cgcttcgctg
      421 attaattacc ccagaaataa ggctaaaaaa ctaatcgcat tatcatccta tggttgttaa
      481 tttgattcgt tcatttgaag gtttgtgggg ccaggttact gccaattttt cctcttcata
      541 accataaaag ctagtattgt agaatcttta ttgttcggag cagtgcggcg cgaggcacat
      601 ctgcgtttca ggaacgcgac cggtgaagac gaggacgcac ggaggagagt cttccttcgg
      661 agggctgtca cccgctcggc ggcttctaat ccgtggacta gtggatcgat ccccacgcgc
      721 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
      781 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
      841 ccggctttcc ccgtcaagct ctaaatcggg g
//
