LOCUS       sequence-73170-e         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains expression cassette for sgRNA targeting 
            tdTomato..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, 
            Thomson JA
  TITLE     Efficient genome engineering in human pluripotent stem cells using 
            Cas9 from Neisseria meningitidis.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 12.
  PUBMED    23940360
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Aug 12."
COMMENT     Sequence Label: pSTBlue-U6-sgRNA(tdTomato)
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        116..364
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     116..136
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     287..306
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        417..509
                     /label=Nm tracrRNA
                     /note="trans-activating CRISPR RNA for the Neisseria 
                     meningitidis CRISPR/Cas9 system (Hou et al., 2013)"
     promoter        complement(607..625)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(608..625)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(639..656)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(639..655)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(648..670)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(933..954)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 acgactcact atagggaaag ctcggtacca cgcatgctgc agacgcgtta cgtatcggat
       61 ccagaattcg tgatccaagc tcggatccga tatcgacgtc aaggtcgggc aggaagaggg
      121 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
      181 tagaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
      241 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
      301 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa
      361 caccgtacgt gaagcacccc gccgacatgt tgtagctccc tttctcattt cggaaacgaa
      421 atgagaaccg ttgctacaat aaggccgtct gaaaagatgt gccgcaacgc tctgcccctt
      481 aaagcttctg ctttaagggg catcgtttat ttcggttttt ttgcatctga attcgtcgac
      541 aagcttctcg agcctaggct agctctagac cacacgtgtg ggggcccgag ctcgcggccg
      601 ctgtattcta tagtgtcacc taaatggccg cacaattcac tggccgtcgt tttacaacgt
      661 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      721 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      781 ctgaatggcg aatggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
      841 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
      901 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
      961 agaacgtgga ctc
//
