LOCUS       sequence-73172-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning Saccharomyces cerevisiae expression vector with GAL1 
            promoter and N-terminal 10x His tag, GFP and 3C protease cleavage 
            site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pYEXNHG3
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(118..141)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(148..858)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             complement(859..888)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     primer_bind     complement(910..929)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(911..929)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
       61 ggaatataaa tgtcaggctc ccttatacac agccagtctg cagctcgctc ttctacttgg
      121 accttgaaac aaaacttcta aactagtttt gtacaattca tccataccat gggtaatacc
      181 agcagcagta acaaattcta acaagaccat gtggtctctc ttttcgtttg gatctttgga
      241 taaggcagat tgagtggata agtaatggtt gtctggtaac aagactggac catcaccaat
      301 tggagtattt tgttgataat ggtcagctaa ttgaacagaa ccatcttcaa tgttgtgtct
      361 aattttgaag ttaactttga taccattctt ttgtttgtca gccatgatgt aaacattgtg
      421 agagttatag ttgtattcca atttgtgacc taaaatgtta ccatcttctt taaaatcaat
      481 accttttaat tcgattctat taactaaggt atcaccttca aacttgactt cagctctggt
      541 cttgtagtta ccgtcatctt tgaaaaaaat agttctttct tgaacataac cttctggcat
      601 ggcagacttg aaaaagtcat gttgtttcat atgatctggg tatctcgcaa aacattgaac
      661 accataaccg aaagtagtga ctaaggttgg ccatggaact ggcaatttac cagtagtaca
      721 aataaatttt aaggtcaatt taccgtaagt agcatcacct tcaccttcac cggagacaga
      781 aaatttgtga ccattaacat caccatctaa ttcaaccaaa attgggacaa caccagtgaa
      841 taattcttct cctttagaat gatgatgatg atggtgatga tgatggtgca tggtaccaag
      901 cttaatattc cctatagtga gtcgtattac agctgctagt agtccgatcc
//
