LOCUS       sequence-73175-e        1103 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9n (D10A nickase mutant) from S. pyogenes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F
  TITLE     Genome engineering using the CRISPR-Cas9 system.
  JOURNAL   Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. 
            Epub 2013 Oct 24.
  PUBMED    24157548
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2013.143"; journalName: "Nat Protoc"; date: 
            "2013-11"; volume: "8"; issue: "11"; pages: "2281-308"
COMMENT     Sequence Label: pSpCas9n(BB) (PX460)
FEATURES             Location/Qualifiers
     source          1..1103
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..57)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gaattcttac tttttctttt ttgcctggcc ggcctttttc gtggccgccg gccttttgtc
       61 gcctcccagc tgagacaggt cgatccgtgt ctcgtacagg ccggtgatgc tctggtggat
      121 cagggtggcg tccagcacct ctttggtgct ggtgtacctc ttccggtcga tggtggtgtc
      181 aaagtacttg aaggcggcag gggctcccag attggtcagg gtaaacaggt ggatgatatt
      241 ctcggcctgc tctctgatgg gcttatcccg gtgcttgttg taggcggaca gcactttgtc
      301 cagattagcg tcggccagga tcactctctt ggagaactcg ctgatctgct cgatgatctc
      361 gtccaggtag tgcttgtgct gttccacaaa cagctgtttc tgctcattat cctcggggga
      421 gcccttcagc ttctcatagt ggctggccag gtacaggaag ttcacatatt tggagggcag
      481 ggccagttcg tttcccttct gcagttcgcc ggcagaggcc agcattctct tccggccgtt
      541 ttccagctcg aacagggagt acttaggcag cttgatgatc aggtcctttt tcacttcttt
      601 gtagcccttg gcttccagaa agtcgatggg attcttctcg aagctgcttc tttccatgat
      661 ggtgatcccc agcagctctt tcacactctt cagtttcttg gacttgccct tttccacttt
      721 ggccaccacc agcacagaat aggccacggt ggggctgtcg aagccgccgt acttcttagg
      781 gtcccagtcc ttctttctgg cgatcagctt atcgctgttc ctcttgggca ggatagactc
      841 tttgctgaag ccgcctgtct gcacctcggt ctttttcacg atattcactt ggggcatgct
      901 cagcactttc cgcacggtgg caaaatcccg gcccttatcc cacacgatct ccccggtttc
      961 gccgtttgtc tcgatcagag gccgcttccg gatctcgccg ttggccaggg taatctcggt
     1021 cttgaaaaag ttcatgatgt tgctgtagaa gaagtacttg gcggtagcct tgccgatttc
     1081 ctgctcgctc ttggcgatca tct
//
