LOCUS       sequence-73176-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Meister G, Landthaler M, Patkaniowska A, Dorsett Y, Teng G, Tuschl T
  TITLE     Human Argonaute2 mediates RNA cleavage targeted by miRNAs and 
            siRNAs.
  JOURNAL   Mol Cell. 2004 Jul 23. 15(2):185-97.
  PUBMED    15260970
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2004 Jul 23. 15(2):185-97."
COMMENT     Sequence Label: pIRESneo-FLAG/HA Ago1
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      94..103
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             103..126
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             127..153
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagcttggta ccgagctcgg atcgatatcg ccggccgcca tggactacaa ggacgacgat
      121 gacaagtacc cttatgacgt gcccgattac gctagcggcc gcatggaagc gggaccctcg
      181 ggagcagctg cgggcgctta cctgcccccc ctgcagcagg tgttccaggc acctcgccgg
      241 cctggcattg gcactgtggg gaaaccaatc aagctcctgg ccaattactt tgaggtggac
      301 atccctaaga tcgacgtgta ccactacgag gtggacatca agccggataa gtgtccccgt
      361 agagtcaacc gggaagtggt ggaatacatg gtccagcatt tcaagcctca gatctttggt
      421 gatcgcaagc ctgtgtatga tggaaagaag aacatttaca ctgtcacagc actgcccatt
      481 ggcaacgaac gggtcgactt tgaggtgaca atccctgggg aagggaagga tcgaatcttt
      541 aaggtctcca tcaagtggct agccattgtg agctggcgaa tgctgcatga ggccctggtc
      601 agcggccaga tccctgttcc cttggagtct gtgcaagccc tggatgtggc catgaggcac
      661 ctggcatcca tgaggtacac ccctgtgggc cgctccttct tctcaccgcc tgagggctac
      721 taccacccgc tggggggtgg gcgcgaggtc tggttcggct ttcaccagtc tgtgcgccct
      781 gccatgtgga agatgatgct caacattgat gtctcagcca ctgcctttta taaggcacag
      841 ccagtgattg agttcatgtg tgaggtgctg gacatcagga acatagatga gcagcccaag
      901 cccctcacgg actctcagcg cgttcgcttc accaaggaga tcaaggggc
//
