LOCUS       sequence-73177-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR
  TITLE     Constitutive Gs activation using a single-construct 
            tetracycline-inducible expression system in embryonic stem cells and
            mice.
  JOURNAL   Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
  PUBMED    21375737
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res Ther. 2011 Mar 4. 2(2):11."
COMMENT     Sequence Label: pcDNA3.2 GW delCMV
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(19..38)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     269..288
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        269..287
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    317..441
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
       61 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
      121 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
      181 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactctgg ctaactagag
      241 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      301 gctagttaag ctatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
      361 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
      421 acaacatatc cagtcactat gaatcaacta cttagatggt attagtgacc tgtagtcgac
      481 cgacagcctt ccaaatgttc ttcgggtgat gctgccaact tagtcgaccg acagccttcc
      541 aaatgttctt ctcaaacgga atcgtcgtat ccagcctact cgctattgtc ctcaatgccg
      601 tattaaatca taaaaagaaa taagaaaaag aggtgcgagc ctcttttttg tgtgacaaaa
      661 taaaaacatc tacctattca tatacgctag tgtcatagtc ctgaaaatca tctgcatcaa
      721 gaacaatttc acaactctta tacttttctc ttacaagtcg ttcggcttca tctggatttt
      781 cagcctctat acttactaaa cgtgataaag tttctgtaat ttctactgta tcgacctgca
      841 gactggctgt gtataaggga gcctgac
//
